Semi Prep HPLC Column, Bidentate C8, 4um, 100A. 10mm ID x 150mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑SP150 Cogent Bidentate C8™ Semi‑Preparative HPLC Column is a long‑format semi‑prep purification column designed for applications requiring high‑resolution fraction collection and improved separation of closely eluting compounds. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, rapid equilibration, and very robust column lifetime under demanding purification workflows.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This creates a phase that is more hydrophobic than conventional C8 columns, yet less hydrophobic than Bidentate C18™, making it ideal when C18 is overly retentive for target analytes. The result is balanced retention well suited for hydrophobic molecules, metabolites, and amine‑containing analytes in preparative workflows.
The 10 mm internal diameter supports large injection volumes and high mass loading, while the extended 150 mm length increases plate count and separation efficiency, enabling higher purity fraction collection compared with shorter semi‑prep formats. This makes the column especially valuable when closely related compounds must be separated prior to collection.
System Note: A specialized semi‑preparative HPLC system (appropriate flow‑rate capability, injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size.
Why Choose the 10 × 150 mm Semi‑Prep Bidentate C8™ Format
This configuration is commonly selected when:
C18 columns are too retentive for preparative targets
Higher resolution is required than 10 × 50 mm or 10 × 100 mm formats provide
Large sample volumes must be purified with improved separation
Hydrophobic or basic analytes require moderated retention behavior
Fraction purity is critical prior to collection
The 150 mm length provides increased interaction time and plate count, while TYPE‑C™ silica hydride chemistry ensures fast equilibration and long service life.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase chemistry
Reduced silanol activity and improved peak shape
Faster equilibration than conventional prep C8 columns
Very robust lifetime for repeated purification runs
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention
Ideal alternative when C18 is overly retentive
Semi‑Preparative Geometry for Maximum Resolution
10 mm ID × 150 mm length
Increased plate count vs. shorter semi‑prep columns
Supports tighter fraction cuts and improved purity
Broad Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Suitable for demanding purification conditions
Typical Applications
Semi‑preparative purification
High‑purity fraction collection
Hydrophobic molecule isolation
Metabolite purification
Amine‑containing analytes
Preparative workflows where C18 retention is excessive
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable